Rabbit anti-Matriptase/ST14 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-221A maps to a region between residues 800 and the C-terminus (residue 855) of human Membrane-type serine protease 1 using the numbering given in SwissProt entry Q9Y5Y6 (GeneID 6768).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Matriptase/ST14 immobilized on solid support.
The epitope recognized by A300-221A-T maps to a region between residues 800 and the C-terminus (residue 855) of human Membrane-type serine protease 1 using the numbering given in SwissProt entry Q9Y5Y6 (GeneID 6768).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Matriptase/ST14 is an epithelial-derived type II transmembrane trypsin-like serine protease that has been shown to cleave and activate various proteases and growth factors. The overexpression of Matriptase /ST14 in a variety of tumors, and its ability to degrade extracellular matrix suggests a role in tumor invasion.
Alternate Names
ARCI11; HAI; Matriptase; membrane-type serine protease 1; MTSP1; MT-SP1; prostamin; PRSS14; serine protease 14; serine protease TADG-15; SNC19; suppression of tumorigenicity 14 (colon carcinoma); suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin); suppressor of tumorigenicity 14 protein; TADG15; TMPRSS14; tumor associated differentially expressed gene 15 protein; Tumor-associated differentially-expressed gene 15 protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.