Rabbit anti-MBD4 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
MBD4 (methyl-CpG-binding domain protein 4) is a member of the MBD family of proteins that mainly functions to bind methylated CpG sites and to repress gene transcription. Unlike its family members, MBD4 exhibits thymine glycosylase activity and has been shown to play an important role in DNA mismatch repair and apoptosis.
Alternate Names
3,N(4)-ethenocytosine glycosylase; G/5-fluorouracil mismatch glycosylase with biphasic kinetics; G/T mismatch glycosylase; G/U mismatch glycosylase; MED1; methyl-CpG binding domain protein 4; methyl-CpG-binding domain protein 4; methyl-CpG-binding endonuclease 1; methyl-CpG-binding protein MBD4; mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.