Rabbit anti-MCAK Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-807A maps to a region between residue 550 and 600 of human Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_006836.1 (GeneID 11004).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MCAK immobilized on solid support.
The epitope recognized by A300-807A-T maps to a region between residue 550 and 600 of human Mitotic Centromere-Associated Kinesin using the numbering given in entry NP_006836.1 (GeneID 11004).
Additional Product Information
Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, chromosome congression, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. MCAK (Mitotic Centromere-Associated Kinesin) is the founding member of kinesin-13 proteins. There are 3 independent genes coding for kinesin-13 proteins kif2a, kif2b, and kif2c (MCAK). Instead of functioning as a translocator of microtubules MCAK is a depolymerizer that is primarily responsible for releasing improper microtubule-kinetochore attachments during cell division.
Alternate Names
CT139; kinesin-like protein 6; kinesin-like protein KIF2C; KNSL6; MCAK; mitotic centromere-associated kinesin; testis tissue sperm-binding protein Li 68n
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.