Rabbit anti-MCEF Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-538A-T maps to a region between residue 50 and 100 of human major CDK9 elongation factor-associated protein of human using the numbering given in entry NP_055238.1 (GeneID 27125).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MCEF immobilized on solid support.
The epitope recognized by A302-538A maps to a region between residue 50 and 100 of human major CDK9 elongation factor-associated protein of human using the numbering given in entry NP_055238.1 (GeneID 27125).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Major CDK9 elongation factor-associated protein (MCEF) is also known as AFF4 AF4/FMR2 family member 4. This family of proteins includes the AF4 proto-oncogene and FMR2 (fragile X E mental retardation syndrome) nuclear proteins. MCEF is a component of the cyclin-dependent kinase pair (CDK9/cyclin T1) complex. A chromosomal aberration involving AFF4 is found in acute lymphoblastic leukemia (ALL).
Alternate Names
AF4/FMR2 family member 4; AF5Q31; ALL1-fused gene from chromosome 5q31 protein; CHOPS; major CDK9 elongation factor-associated protein; MCEF; Protein AF-5q31
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.