Rabbit anti-MCM3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-192A maps to a region between residues 500 and 550 of human Minichromosome Maintenance 3 using the numbering given in entry NP_002379.2 (GeneID 4172).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MCM3 immobilized on solid support.
The epitope recognized by A300-192A-T maps to a region between residues 500 and 550 of human Minichromosome Maintenance 3 using the numbering given in entry NP_002379.2 (GeneID 4172).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM3 has been shown to interact with MCM5, CDC46, and the acetyltransferase MCM3AP.
Alternate Names
cervical cancer proto-oncogene 5; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor MCM3; HCC5; hRlf beta subunit; MCM3 minichromosome maintenance deficient 3; minichromosome maintenance deficient 3; P1.h; p102; P1-MCM3; replication licensing factor, beta subunit; RLF subunit beta; RLFB
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.