Rabbit anti-MCM6 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-194A-T maps to a region between residue 775 and the C-terminus (residue 821) of human Minichromosome Maintenance 6 using the numbering given in entry NP_005906.2 (GeneID 4175). Antibody was affinity purified using an epitope specific to MCM6 immobilized on solid support.
The epitope recognized by A300-194A maps to a region between residue 775 and the C-terminus (residue 821) of human Minichromosome Maintenance 6 using the numbering given in entry NP_005906.2 (GeneID 4175).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM6 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity.
Alternate Names
DNA replication licensing factor MCM6; MCG40308; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); minichromosome maintenance deficient (mis5, S. pombe) 6; Mis5; P105MCM
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).