Rabbit anti-MED1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-793A maps to a region between residue 1525 and the C-terminus (residue 1581) of human Mediator complex subunit 1 using the numbering given in entry NP_004765.2 (GeneID 5469).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MED1 immobilized on solid support.
The epitope recognized by A300-793A-T maps to a region between residue 1525 and the C-terminus (residue 1581) of human Mediator comlex subunit 1 using the numbering given in entry NP_004765.2 (GeneID 5469).
Additional Product Information
MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q15648].
Alternate Names
activator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; Mediator complex subunit 1; mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; peroxisome proliferator-activated receptor-binding protein; PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyroid receptor-interacting protein 2; TRAP220; TR-interacting protein 2; TRIP2; TRIP-2; vitamin D receptor-interacting protein 230 kD
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.