Rabbit anti-MEK2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-141A maps to a region between residue 1 and 50 of human MAPK/ERK kinase 2 using the numbering given in entry NP_109587.1 (GeneID 5605).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MEK2 immobilized on solid support.
The epitope recognized by A302-141A-T maps to a region between residue 1 and 50 of human MAPK/ERK kinase 2 using the numbering given in entry NP_109587.1 (GeneID 5605).
Additional Product Information
MEK2 (MAP2K2) is a dual specificity protein kinase that belongs to the MAP kinase kinase family. MEK2 is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3, and is involved in a multitude of cellular processes such as proliferation, differentiation, gene activation, and development [taken from NCBI Entrez Gene (Gene ID: 5605)].
Alternate Names
CFC4; dual specificity mitogen-activated protein kinase kinase 2; ERK activator kinase 2; MAP kinase kinase 2; MAPK/ERK kinase 2; MAPKK 2; MAPKK2; MEK 2; MEK2; mitogen-activated protein kinase kinase 2, p45; MKK2; PRKMK2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.