Rabbit anti-MetAP2 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Methionine aminopeptidase 2 (MetAP) removes the amino-terminal methionine from nascent proteins. The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P50579].
Alternate Names
eIF-2-associated p67 homolog; initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MetAP 2; methionine aminopeptidase 2; MNPEP; p67; p67eIF2; Peptidase M; peptidase M 2; testicular tissue protein Li 17
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.