Rabbit anti-METTL3/MT-A70 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-567A maps to a region between residue 1 and 50 of human methyltransferase like 3 (N6-adenosine-methyltransferase 70 kDa subunit) using the numbering given in entry NP_062826.2 (GeneID 56339).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to METTL3/MT-A70 immobilized on solid support.
The epitope recognized by A301-567A-T maps to a region between residue 1 and 50 of human methyltransferase like 3 (N6-adenosine-methyltransferase 70 kDa subunit) using the numbering given in entry NP_062826.2 (GeneID 56339).
Additional Product Information
METTL3/MT-A70 is a subunit of the N6-methyltransferase that specifically methylates adenines in pre-mRNAs.
Alternate Names
adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; hMETTL3; IME4; M6A; methyltransferase-like protein 3; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; MT-A70; N6-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase catalytic subunit; Spo8
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.