Rabbit anti-MLL1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-087A maps to a region between residues 1320 and 1380 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297). The epitope is found in the N-terminal 300 kDa fragment generated by proteolytic cleavage. The epitope is not found in isoform 14P-18B of MLL1.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MLL1 immobilized on solid support.
The epitope recognized by A300-087A-T maps to a region between residues 1320 and 1380 of human myeloid/lymphoid or mixed-lineage leukemia 1 using the number given in Swiss-Prot entry Q03164 (GeneID 4297). The epitope is found in the N-terminal 300 kDa fragment generated by proteolytic cleavage. The epitope is not found in isoform 14P-18B of MLL1.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
MLL1 (mixed lineage leukemia 1) has been found to participate in a multitude of chromosomal translocations that are associated with a variety of hematologic malignancies. MLL1 functions as a transcriptional regulator and plays and important role in HOX gene expression during embryonic development.
Alternate Names
ALL-1; CXXC7; CXXC-type zinc finger protein 7; histone-lysine N-methyltransferase 2A; HRX; HTRX1; lysine (K)-specific methyltransferase 2A; lysine N-methyltransferase 2A; mixed lineage leukemia 1; MLL; MLL1; MLL1A; Myeloid/lymphoid or mixed-lineage leukemia; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); Myeloid/lymphoid or mixed-lineage leukemia protein 1; trithorax-like protein; TRX1; WDSTS; zinc finger protein HRX
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.