Rabbit anti-MondoA Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-195A maps to a region between residue 869 and 919 of human Transcriptional Activator MondoA using the numbering given in entry NP_055753.3 (GeneID 22877).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MondoA immobilized on solid support.
The epitope recognized by A303-195A-T maps to a region between residue 869 and 919 of human Transcriptional Activator MondoA using the numbering given in entry NP_055753.3 (GeneID 22877).
Additional Product Information
MondoA binds DNA as a heterodimer with MLX and activates transcription. MondoA binds to the canonical E box sequence 5'-CACGTG-3'. It plays a role in transcriptional activation of glycolytic target genes and is involved in glucose-responsive gene regulation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9HAP2].
Alternate Names
bHLHe36; class E basic helix-loop-helix protein 36; MIR; Mlx interactor; MLX-interacting protein; MONDOA; transcriptional activator MondoA
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.