Rabbit anti-MST1/STK4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-465A maps to a region between residues 375 and 425 of human Mammalian Sterile 20-like 1 (Serine/Threonine Kinase 4) using the numbering given in Swiss Prot entry Q13043 (GeneID 6789).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
MST1 is a member of the sterile 20 (Ste20)-like kinase family that acts upstream of mitogen-activated protein kinase (MAPK) pathways and regulates c-Jun N-terminal kinase (JNK), p38 and extracellular signal-regulated kinase (ERK). Ste20-like kinases are involved in a variety of cellular processes which include gene transcription, stress responses, cytoskeletal organization and apoptosis. MST1 can phosphorylate myelin basic protein and FOXO3. MST1 has been identified as a substrate of caspase and is required to mediate apoptosis in response to various apoptotic stimuli.
Alternate Names
kinase responsive to stress 2; KRS2; mammalian STE20-like protein kinase 1; mammalian sterile 20-like 1; MST1; MST-1; serine/threonine-protein kinase 4; serine/threonine-protein kinase Krs-2; STE20-like kinase MST1; YSK3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.