Rabbit anti-MST3/STK24 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-564A maps to a region between residues 325 and 375 of human Mammalian Ste20-like Kinase 3 (Serine/Threonine Kinase 24) using the numbering given in entry NP_003567.2 (GeneID 8428).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to MST3/STK24 immobilized on solid support.
The epitope recognized by A300-564A-T maps to a region between residues 325 and 375 of human Mammalian Ste20-like Kinase 3 (Serine/Threonine Kinase 24) using the numbering given in entry NP_003567.2 (GeneID 8428).
Additional Product Information
MST3/STK24 is a member of the sterile 20 (Ste20)-like kinase family that acts upstream of mitogen-activated protein kinase (MAPK) pathways and regulates c-Jun N-terminal kinase (JNK), p38 and extracellular signal-regulated kinase (ERK). Ste20-like kinases are involved in a variety of cellular processes which include gene transcription, stress responses, cytoskeletal organization and apoptosis. In response to apoptotic signals, MST3/STK24 is cleaved by caspase-3 and mediates apoptosis. The biological functions of MST3/STK24 are not well understood.
Alternate Names
epididymis secretory protein Li 95; HEL-S-95; mammalian STE20-like protein kinase 3; MST3; MST-3; MST3B; serine/threonine kinase 24 (STE20 homolog, yeast); serine/threonine-protein kinase 24; STE20; STE20-like kinase 3; STE20-like kinase MST3; sterile 20-like kinase 3; STK3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.