Rabbit anti-NAMPT/PBEF/Visfatin Recombinant Monoclonal Antibody [BLR058F]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 400 and 450 of human Nicotinamide phosphoribosyltransferase precursor (NAMPT), using the numbering given in entry NP_005737.1 (Gene ID 10135).
Additional Product Information
PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar.
Alternate Names
1110035O14Rik; NAmPRTase; Nampt; nicotinamide phosphoribosyltransferase; PBEF; PBEF1; pre-B cell-enhancing factor; pre-B-cell colony-enhancing factor 1; VF; VISFATIN
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.