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Rabbit anti-NEK1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
NEK1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1208 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to NEK1 immobilized on solid support.

The epitope recognized by A304-570A maps to a region between residue 1208 to 1258 of human Never in Mitosis A-Related Kinase 1 using the numbering given in entry NP_036356.1 (GeneID 4750).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to NEK1 immobilized on solid support.

The epitope recognized by A304-570A-T maps to a region between residue 1208 to 1258 of human Never in Mitosis A-Related Kinase 1 using the numbering given in entry NP_036356.1 (GeneID 4750).
Country of Origin
USA

Additional Product Information

Never in mitosis A-related kinase 1 (NEK1) is a serine/threonine kinase involved in cell cycle regulation. NEK1 is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD) [taken from NCBI Entrez Gene (Gene ID: 4750)].

4750
NEK1
NIMA related kinase 1
Q96PY6
Serine/threonine-protein kinase Nek1

Alternate Names

ALS24; never in mitosis A-related kinase 1; NIMA (never in mitosis gene a)-related kinase 1; nimA-related protein kinase 1; NY-REN-55; protein-serine/threonine kinase; renal carcinoma antigen NY-REN-55; serine/threonine-protein kinase Nek1; SRPS2; SRPS2A; SRTD6

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).