Rabbit anti-NEK7 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-684A maps to a region between residue 1 and 50 of human NIMA (never in mitosis gene a)-related kinase 7 using the numbering given in entry NP_598001.1 (GeneID 140609).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NEK7 immobilized on solid support.
The epitope recognized by A302-684A-T maps to a region between residue 1 and 50 of human NIMA (never in mitosis gene a)-related kinase 7 using the numbering given in entry NP_598001.1 (GeneID 140609).
Additional Product Information
NEK7 [NIMA(never in mitosis gene a)-related kinase 7] is a member of the NIMA-related serine/threonine kinase family. NEK7 has been shown localize to spindle poles and to be required for mitotic spindle formation and cytokinesis.
Alternate Names
never in mitosis A-related kinase 7; NIMA (never in mitosis gene a)-related kinase 7; nimA-related protein kinase 7; serine/threonine-protein kinase Nek7
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.