Rabbit anti-NELFA Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-910A maps to a region between residue 499 and 549 of human negative elongation factor A using the numbering given in entry BAB18651.1 (GeneID 7469).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NELFA immobilized on solid support.
The epitope recognized by A301-910A-T maps to a region between residue 499 and 549 of human negative elongation factor A using the numbering given in entry BAB18651.1 (GeneID 7469).
Additional Product Information
NELFA (negative elongation factor A) is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF, along with DRB sensitivity-inducing factor (DSIF), functions to cause transcriptional pausing of RNA polymerase II. NELFA may play a role in Wolf-Hirschhorn syndrome (WHS).
Alternate Names
negative elongation factor A; NELF-A; P/OKcl.15; WHSC2; wolf-Hirschhorn syndrome candidate 2 protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.