Rabbit anti-Neurofibromin 1 Recombinant Monoclonal Antibody [BLR345M]
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 2789 and the C-terminus (residue 2839) of human Neurofibromin 1 using the numbering given in entry NP_001035957.1 (Gene ID 4763).
Additional Product Information
Inactivating mutations in NF1 (neurofibromin 1) result in neurofibromatosis type I (NF1) which is characterized by Schwann cell neurofibromas, café-au-lait spots, and benign lesions of the iris. At the cellular level, NF1 functions as a negative regulator of Ras activity. Loss of NF1 leads to increased levels of active Ras-GTP which is important for the formation and maintenance of Schwann cell tumors.
Alternate Names
neurofibromatosis 1; neurofibromatosis-related protein NF-1; neurofibromin; NFNS; truncated neurofibromin 1; VRNF; WSS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.