Rabbit anti-NF-kappaB2 (p100) Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-821A maps to a region between residue 810 and 860 of human nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) using the numbering given in entry NP_001070962.1 (GeneID 4791).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NF-kappaB2 immobilized on solid support.
The epitope recognized by A301-821A-T maps to a region between residue 810 and 860 of human nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) using the numbering given in entry NP_001070962.1 (GeneID 4791).
Additional Product Information
The NFKB2 gene encodes a 100 kDa protein that can undergo co-translational processing to also yield a 52 kDa protein. The 52 kDa protein is also known as NF-kappa B2/p52 and is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. The 100 kDa product is also known as p100 or I kappa B delta. P100/I kappa B delta has been shown to function as an inhibitor protein that interacts with NF-kappa B and inhibit its DNA binding activity. P100/I kappa B delta is a member of the I kappa B family of proteins which includes I kappa B-beta, I kappa B-delta/NF-kappa B2/p100, I kappa B-gamma/NF-kappa B1/p105, I kappa B-epsilon, I kappa B-zeta, and Bcl3. The classical description of the NF-kappa B transcription factor is a transcription factor that reprograms gene expression of the immune system in response to injury, infection, and stress.
Alternate Names
CVID10; DNA-binding factor KBF2; H2TF1; lymphocyte translocation chromosome 10 protein; LYT10; LYT-10; NFKB, p52/p100 subunit; NF-kB2; nuclear factor Kappa-B, subunit 2; nuclear factor NF-kappa-B p100 subunit; nuclear factor NF-kappa-B p52 subunit; nuclear factor of Kappa light chain gene enhancer in B cells 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); oncogene Lyt-10; p100; p49/p100; p52; transcription factor NFKB2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.