Rabbit anti-NF45 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-147A maps to a region between residue 340 and 390 of human Nuclear Factor of Activated T-cells 45 kDa using the numbering given in entry NP_004506.2 (GeneID 3608).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NF45 immobilized on solid support.
The epitope recognized by A303-147A-T maps to a region between residue 340 and 390 of human Nuclear Factor of Activated T-cells 45 kDa using the numbering given in entry NP_004506.2 (GeneID 3608).
Additional Product Information
The 45kDa nuclear factor of activated T-cells (NF45) functions as part of a heterodimeric complex with NF90 to form the transcription factor, NFAT (nuclear factor of activated T-cells). The NF45/NF90 complex binds the interleukin-2 promoter and regulates transcription of the IL2 gene during T-cell activation. It has also been shown that the NF45/NF90 complex can function as a negative regulator of miRNA biogenesis.
Alternate Names
interleukin enhancer binding factor 2, 45kD; interleukin enhancer binding factor 2, 45kDa; interleukin enhancer-binding factor 2; NF45; Nuclear factor of activated T-cells 45 kDa; nuclear factor of activated T-cells, 45-kDa; PRO3063
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.