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Primary Antibodies

Rabbit anti-NHP2L1 Antibody Affinity Purified

Name: Rabbit anti-NHP2L1 Antibody
Brand: Bethyl Laboratories
SKU: A304-030A
Price: 131 USD

Bethyl Laboratories Catalog #

Citations (1)

Target:

NHP2L1

Reactivity:

Human, Mouse

Applications:

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 78 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00

Qty:

Product Details

Specifications

Verified Reactivity

Mouse, Human

Presumed Reactivity

X. tropicalis, Bovine, Rat

Antigen Species

Human

Concentration

1000 µg/ml

Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State

Liquid

Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures

Antibody was affinity purified using an epitope specific to NHP2L1 immobilized on solid support.

The epitope recognized by A304-030A-T maps to a region between residue 71 to 121 of human NHP2 Non-Histone Chromosome Protein 2-Like 1 using the numbering given in entry NP_004999.1 (GeneID 4809).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NHP2L1 immobilized on solid support.

The epitope recognized by A304-030A maps to a region between residue 71 to 121 of human NHP2 Non-Histone Chromosome Protein 2-Like 1 using the numbering given in entry NP_004999.1 (GeneID 4809).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

NHP2 non-histone chromosome protein 2-like 1 (NHP2L1) was originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene [taken from NCBI Entrez Gene (Gene ID: 4809)].

Gene ID: 4809

Gene Symbol: SNU13

Gene Name: small nuclear ribonucleoprotein 13

Uniprot ID: P55769

Protein Name: NHP2-like protein 1

Alternate Names

U4/U6.U5 tri-snRNP 15.5 kD RNA binding protein; 15.5K; FA1; FA-1; high mobility group-like nuclear protein 2 homolog 1; hSNU13; NHP2 non-histone chromosome protein 2-like 1; NHP2L1; NHP2-like protein 1; NHPX; non-histone chromosome protein 2-like 1; OTK27; SNRNP15-5; SNU13 homolog; SNU13 homolog, small nuclear ribonucleoprotein (U4/U6.U5); SPAG12; sperm specific antigen 1; SSFA1; U4/U6.U5 small nuclear ribonucleoprotein SNU13; U4/U6.U5 tri-snRNP 15.5 kDa protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.