Rabbit anti-NIP7 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-974A maps to a region between residue 130 to 180 of human Nuclear Import 7 using the numbering given in entry NP_057185.1 (GeneID 51388).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NIP7 immobilized on solid support.
The epitope recognized by A303-974A-T maps to a region between residue 130 to 180 of human Nuclear Import 7 using the numbering given in entry NP_057185.1 (GeneID 51388).
Additional Product Information
Nuclear import 7 homolog (NIP7) interacts with pre-ribosome complex and is required for proper 34S pre-rRNA processing and 60S ribosome subunit assembly [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y221].
Alternate Names
60S ribosome subunit biogenesis protein NIP7 homolog; CGI-37; HSPC031; KD93; nuclear import 7 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.