Rabbit anti-NOL12 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-733A maps to a region between residue 163 and 213 of human Nucleolar Protein 12 using the numbering given in entry NP_077289.1 (GeneID 79159).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NOL12 immobilized on solid support.
The epitope recognized by A302-733A-T maps to a region between residue 163 and 213 of human Nucleolar Protein 12 using the numbering given in entry NP_077289.1 (GeneID 79159).
Additional Product Information
Nucleolar protein 12 (NOL12) is a member of the RRP17 (ribosomal RNA-processing protein 17) family of proteins. NOL12 is inferred to be an RNA binding protein that binds 28S rRNA and localizes to the nucleolus. The function of NOL12 has not been characterized.
Alternate Names
dJ37E16.7; Nop25; nucleolar protein 12
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.