Rabbit anti-NOM1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-844A maps to a region between residue 200 and 250 of human Nucleolar MIF4G domain-containing protein 1 using the numbering given in entry Q5C9Z4.1 (GeneID 64434).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NOM1 immobilized on solid support.
The epitope recognized by A305-844A-T maps to a region between residue 200 and 250 of human Nucleolar MIF4G domain-containing protein 1 using the numbering given in entry Q5C9Z4.1 (GeneID 64434).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
NOM1 plays a role in targeting PPP1CA to the nucleolus. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5C9Z4].
Alternate Names
C7orf3; nucleolar MIF4G domain-containing protein 1; PPP1R113; protein phosphatase 1, regulatory subunit 113; SGD1; SGD1 homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.