Rabbit anti-NPM1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-402A maps to a region between residue 100 and 150 of human nucleophosmin using the numbering given in entry NP_002511.1 (GeneID 4869).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NPM1 immobilized on solid support.
The epitope recognized by A302-402A-T maps to a region between residue 100 and 150 of human nucleophosmin using the numbering given in entry NP_002511.1 (GeneID 4869).
Additional Product Information
Nucleophosmin (NPM1) is involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors TP53/p53 and ARF. It associates with nucleolar ribonucleoprotein structures and binds single-stranded nucleic acids. NPM1 binds ribosomes presumably to drive ribosome nuclear export. Chromosomal aberrations involving NPM1 have been identified in several types of leukemia [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P06748].
Alternate Names
B23; NPM; Nucleolar phosphoprotein B23; nucleolar protein NO38; nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin/nucleoplasmin family, member 1; Numatrin; testicular tissue protein Li 128
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).