Rabbit anti-NSFL1C Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-565A maps to a region between residue 25 to 75 of human NSFL1 (p97) Cofactor (p47) using the numbering given in entry NP_057227.2 (GeneID 55968).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NSFL1C immobilized on solid support.
The epitope recognized by A304-565A-T maps to a region between residue 25 to 75 of human NSFL1 (p97) Cofactor (p47) using the numbering given in entry NP_057227.2 (GeneID 55968).
Additional Product Information
N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of NSFL1C binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments [taken from NCBI Entrez Gene (Gene ID: 55968)].
Alternate Names
dJ776F14.1; NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; P47; p97 cofactor p47; SHP1 homolog; UBX domain-containing protein 2C; UBX1; UBXD10; UBXN2C
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.