Rabbit anti-Nucleolin (NCL) Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-711A maps to a region between residue 550 and the C-terminus (residue 710) of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Nucleolin (NCL) immobilized on solid support.
The epitope recognized by A300-711A-T maps to a region between residue 550 and the C-terminus (residue 710) of human Nucleolin using the numbering given in entry NP_005372.2 (GeneID 4691).
Additional Product Information
Nucleolin (NCL) is a major nucleolar phosphoprotein that is involved in pre-RNA transcription and the synthesis and maturation of ribosomes. NCL can bind to histone H1 to induce chromatin condensation and may play an additional role in transcriptional elongation.
Alternate Names
C23; Nsr1; nucleolin; Protein C23
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.