Rabbit anti-NUP153 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-788A maps to a region between residue 50 and 100 of human nucleoporin 153kDa using the numbering given in entry NP_005115.2 (GeneID 9972).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NUP153 immobilized on solid support.
The epitope recognized by A301-788A-T maps to a region between residue 50 and 100 of human nucleoporin 153kDa using the numbering given in entry NP_005115.2 (GeneID 9972).
Additional Product Information
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. About 30 different human Nups have been identified and appear to serve non-redundant functions. Nup153 contains four RanBP2-type zinc fingers and an RNA binding domain. The zinc-fingers of Nup153 can bind the small GTPase, Ran. The RNA binding domain has been shown to have an affinity for single-stranded RNA.
Alternate Names
153 kDa nucleoporin; HNUP153; N153; nuclear pore complex protein hnup153; nuclear pore complex protein Nup153; nucleoporin 153kD; nucleoporin 153kDa; nucleoporin Nup153
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.