Rabbit anti-NUP85 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-978A maps to a region between residue 1 to 50 of human Nucleoporin 85kDa using the numbering given in entry NP_079120.1 (GeneID 79902).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NUP85 immobilized on solid support.
The epitope recognized by A303-978A-T maps to a region between residue 1 to 50 of human Nucleoporin 85kDa using the numbering given in entry NP_079120.1 (GeneID 79902).
Additional Product Information
Nucleoporin 85 (NUP85) is an essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex, it plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2 and seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BW27].
Alternate Names
85 kDa nucleoporin; FROUNT; nuclear pore complex protein Nup85; nucleoporin 85kDa; nucleoporin Nup75; nucleoporin Nup85; Nup75; Pericentrin-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.