Rabbit anti-NUP98 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-786A maps to a region between residue 600 and 650 of human nucleoporin 98kDa using the numbering given in entry NP_057404.2 (GeneID 4928).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to NUP98 immobilized on solid support.
The epitope recognized by A301-786A-T maps to a region between residue 600 and 650 of human nucleoporin 98kDa using the numbering given in entry NP_057404.2 (GeneID 4928).
Additional Product Information
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. There are about 30 different human Nups that have been identified. Nup98 is a 98 kDa protein generated from a 186 kDa precursor. The proteolytic cleavage of the 186 kDa precursor also results in a 96 kDa nucleoporin, Nup96. Nup98 and Nup96 have been shown to interact with each other and both play a role in bidirectional transport across the NPC. The NUP98 gene localizes to tumor-suppressor region associated with several types of cancer.
Alternate Names
ADIR2; GLFG-repeat containing nucleoporin; nuclear pore complex protein Nup98; nuclear pore complex protein Nup98-Nup96; nucleoporin 98kD; nucleoporin 98kDa; NUP196; NUP96; NUP98/PHF23 fusion 2 protein; Nup98-Nup96
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.