Rabbit anti-OGFOD1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-812A maps to a region between residue 1 and 50 of human 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1 using the numbering given in entry Q8N543.1 (GeneID 55239).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to OGFOD1 immobilized on solid support.
The epitope recognized by A305-812A-T maps to a region between residue 1 and 50 of human 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1 using the numbering given in entry Q8N543.1 (GeneID 55239).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
OGFOD1 is a prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit uS12 (RPS23), thereby regulating protein translation termination efficiency. Involved in stress granule formation. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N543].
Alternate Names
2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1; prolyl 3-hydroxylase OGFOD1; termination and polyadenylation 1 homolog; TPA1; TPA1, termination and polyadenylation 1, homolog; uS12 prolyl 3-hydroxylase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.