Rabbit anti-OTUB1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
OTUB1 (OTU domain, ubiquitin aldehyde binding 1) is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth [taken from NCBI Entrez Gene (Gene ID: 55611].
Alternate Names
deubiquitinating enzyme OTUB1; hOTU1; HSPC263; OTB1; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU1; otubain-1; OTU-domain Ubal-binding 1; ubiquitin thioesterase OTUB1; ubiquitin-specific protease otubain 1; ubiquitin-specific-processing protease OTUB1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.