Rabbit anti-p21/CIP1/CDKN1A Recombinant Monoclonal Antibody [BLR337M]
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 100 and 150 of human cyclin-dependent kinase inhibitor 1 (aka p21 or CIP1) using the numbering given in entry NP_000380.1 (Gene ID 1026).
Additional Product Information
p21/CIP1/CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression of p21/CIP1/CDKN1A is tightly controlled by the tumor suppressor protein p53, and it mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. p21/CIP1/CDKN1A can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. [taken from NCBI Entrez Gene (Gene ID: 1026)].
Alternate Names
CAP20;CDK-interacting protein 1;CDK-interaction protein 1;CDKN1;CIP1;cyclin-dependent kinase inhibitor 1;cyclin-dependent kinase inhibitor 1A (p21, Cip1);DNA synthesis inhibitor;MDA-6;melanoma differentiation associated protein 6;Melanoma differentiation-associated protein 6;P21;p21CIP1;SDI1;WAF1;wild-type p53-activated fragment 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.