Rabbit anti-P4HB/PDI Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-790A maps to a region between residue 50 to 100 of human Prolyl 4-Hydroxylase, Beta Polypeptide using the numbering given in entry P07237 (GeneID 5034).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to P4HB/PDI immobilized on solid support.
The epitope recognized by A304-790A-T maps to a region between residue 50 to 100 of human Prolyl 4-Hydroxylase, Beta Polypeptide using the numbering given in entry P07237 (GeneID 5034).
Additional Product Information
P4HB is a multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, P4HB seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P07237].
Alternate Names
cellular thyroid hormone-binding protein; CLCRP1; collagen prolyl 4-hydroxylase beta; DSI; ERBA2L; GIT; glutathione-insulin transhydrogenase; P4Hbeta; p55; PDI; PDIA1; PHDB; PO4DB; PO4HB; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; PROHB; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase family A, member 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase-associated 1; protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone-binding protein p55
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).