Rabbit anti-p57kip2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-346A-T maps to a region between residue 225 and 275 of human cyclin-dependent kinase inhibitor p57 using the numbering given in entry NP_000067.1 (GeneID 1028).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to p57kip2 immobilized on solid support.
The epitope recognized by A302-346A maps to a region between residue 225 and 275 of human cyclin-dependent kinase inhibitor p57 using the numbering given in entry NP_000067.1 (GeneID 1028).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
p57kip2 is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate [taken from NCBI GeneID:1028].
Alternate Names
BWCR; BWS; cyclin-dependent kinase inhibitor 1C; cyclin-dependent kinase inhibitor 1C (p57, Kip2); cyclin-dependent kinase inhibitor p57; KIP2; p57; p57Kip2; WBS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.