Rabbit anti-p73 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-126A maps to a region between residues 1 and 62 of human tumor protein p73 using the numbering given in Swiss-Prot entry O15350 (GeneID 7161). The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73. The antibody is not expected to react with deltaNp73.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to p73 immobilized on solid support.
The epitope recognized by A300-126A-T maps to a region between residues 1 and 62 of human tumor protein p73 using the numbering given in Swiss-Prot entry O15350 (GeneID 7161). The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73. The antibody is not expected to react with deltaNp73.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
p73 is a p53-related protein and a member of the family of transcription factors which include p53, p63 and p73. Although structurally and functionally related, it appears that the members of this family possess their own unique functions. No human genetic disorders have been linked to germ line mutations of p73; however, loss of heterozygosity has been associated with some cancers although the incidence is rare. Generally speaking, p73 appears to play an important role in gene transactivation and growth suppression. P73 participates in the apoptotic response to DNA damage and may function in connection with p53.
Alternate Names
p53-like transcription factor; p53-related protein; P73; tumor protein p73
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.