Rabbit anti-PAK4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-356A maps to a region between residue 25 and 75 of human p21-Activated Kinase 4 using the numbering given in entry NP_005875.1 (GeneID 10298).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PAK4 immobilized on solid support.
The epitope recognized by A300-356A-T maps to a region between residue 25 and 75 of human p21-Activated Kinase 4 using the numbering given in entry NP_005875.1 (GeneID 10298).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
PAK4 is a member of the PAK family of serine/threonine p21-activating kinases. PAK4 is a target for the GTP-bound Rho GTPase, CDC42, and is an important factor that links Rho GTPases such as Ras to transformation. In this context, PAK4 is highly transforming, and has been found to be highly expressed in some tumor cell lines. PAK4 has been demonstrated to activate the JNK pathway and mediate the formation of filopodia and the reorganization of the actin cytoskeleton. PAK4 has also been found to function as a regulator of prosurvival pathways and senescence.
Alternate Names
p21 protein (Cdc42/Rac)-activated kinase 4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; PAK-4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; serine/threonine-protein kinase PAK 4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.