Rabbit anti-PAM16 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-698A maps to a region between residue 50 to 100 of human Mitochondrial import inner membrane translocase subunit TIM16 using the numbering given in entry Q9Y3D7.2 (GeneID 51025).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PAM16 immobilized on solid support.
The epitope recognized by A305-698A-T maps to a region between residue 50 to 100 of human Mitochondrial import inner membrane translocase subunit TIM16 using the numbering given in entry Q9Y3D7.2 (GeneID 51025).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
PAM16 regulates ATP-dependent protein translocation into the mitochondrial matrix.It inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y3D7].
Alternate Names
CGI-136; MAGMAS; magmas-like protein; mitochondria associated protein involved in granulocyte macrophage colony stimulating factor signal transduction; mitochondria-associated granulocyte macrophage CSF-signaling molecule; mitochondrial import inner membrane translocase subunit TIM16; presequence translocase associated motor 16 homolog; Presequence translocated-associated motor subunit PAM16; SMDMDM; TIM16; TIMM16
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.