Rabbit anti-PAPD1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
PAPD1 (mitochondrial poly(A) polymerase) is a polymerase that creates the 3' poly(A) tail of mitochondrial transcripts. PAPD1 can use all four nucleotides, but has higher activity with ATP and UTP (in vitro). It plays a role in replication-dependent histone mRNA degradation. It may be involved in the terminal uridylation of mature histone mRNAs before their degradation is initiated and might be responsible for the creation of some UAA stop codons which are not encoded in mtDNA [taken from NCBI Entrez Gene (Gene ID: 55149)].
Alternate Names
mtPAP; PAP; PAP-associated domain-containing protein 1; PAPD1; poly(A) RNA polymerase, mitochondrial; polynucleotide adenylyltransferase; SPAX4; TENT6; terminal uridylyltransferase 1; TUTase 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.