Rabbit anti-PBRM1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-591A maps to a region between residue 1639 and 1689 of human polybromo 1 (BRG1-associated factor 180) using the numbering given in entry AAP34197.1 (GeneID 55193).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PB1/BAF180 immobilized on solid support.
The epitope recognized by A301-591A-T maps to a region between residue 1639 and 1689 of human polybromo 1 (BRG1-associated factor 180) using the numbering given in entry AAP34197.1 (GeneID 55193).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PBRM1 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PBRM1 has been proposed to function as a tumor suppressor protein. Recently, PBRM1 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21.
Alternate Names
BAF180; BRG1-associated factor 180; hPB1; PB1; polybromo-1D; protein polybromo-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.