Rabbit anti-PCF11 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-706A maps to a region between residue 1505 and 1555 of human Cleavage and Polyadenylation Factor II Subunit using the numbering given in entry NP_056969.2 (GeneID 51585).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PCF11 immobilized on solid support.
The epitope recognized by A303-706A-T maps to a region between residue 1505 and 1555 of human Cleavage and Polyadenylation Factor II Subunit using the numbering given in entry NP_056969.2 (GeneID 51585).
Additional Product Information
PCF11 (pre-mRNA cleavage complex 2 protein) plays a role in transcriptional termination.
Alternate Names
PCF11, cleavage and polyadenylation factor subunit, homolog; PCF11p homolog; pre-mRNA cleavage complex 2 protein Pcf11; pre-mRNA cleavage complex II protein Pcf11
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.