Rabbit anti-PDE3A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-741A maps to a region between residue 575 and 625 of human Phosphodiesterase 3A using the numbering given in entry NP_000912.3 (GeneID 5139).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PDE3A immobilized on solid support.
The epitope recognized by A302-741A-T maps to a region between residue 575 and 625 of human Phosphodiesterase 3A using the numbering given in entry NP_000912.3 (GeneID 5139).
Additional Product Information
PDE3A (cGMP-inhibited Phosphodiesterase 3A) is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP.
Alternate Names
cAMP phosphodiesterase, myocardial cGMP-inhibited; CGI-PDE; CGI-PDE A; CGI-PDE-A; cGMP-inhibited 3',5'-cyclic phosphodiesterase A; cyclic GMP-inhibited phosphodiesterase A; HTNB; phosphodiesterase 3A, cGMP-inhibited
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.