Rabbit anti-PDE3B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-743A maps to a region between residue 1062 and 1112 of human Phosphodiesterase 3B using the numbering given in entry NP_000913.2 (GeneID 5140).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PDE3B immobilized on solid support.
The epitope recognized by A302-743A-T maps to a region between residue 1062 and 1112 of human Phosphodiesterase 3B using the numbering given in entry NP_000913.2 (GeneID 5140).
Additional Product Information
PDE3B (phosphodiesterase 3B) is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP. PDE3B may play a role in fat metabolism.
Alternate Names
CGIP1; CGI-PDE B; cGIPDE1; cGMP-inhibited 3',5'-cyclic phosphodiesterase B; cyclic GMP-inhibited phosphodiesterase B; cyclic nucleotide phosphodiesterase; HcGIP1; phosphodiesterase 3B, cGMP-inhibited
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.