Rabbit anti-PEG10 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-063A-T maps to a region between residue 325 to 375 of human Retrotransposon-derived protein PEG10 using the numbering given in entry Q86TG7.2 (GeneID 23089).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PEG10 immobilized on solid support.
The epitope recognized by A305-063A maps to a region between residue 325 to 375 of human Retrotransposon-derived protein PEG10 using the numbering given in entry Q86TG7.2 (GeneID 23089).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Retrotransposon-derived protein PEG10 prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. PEG10 may also have a role in cell growth promotion and hepatoma formation. It inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1 [taken from UniProtKB (Entry: Q86TG7)].
Alternate Names
EDR; embryonal carcinoma differentiation regulated; Embryonal carcinoma differentiation-regulated protein; HB-1; mammalian retrotransposon-derived 2; mammalian retrotransposon-derived protein 2; Mar2; Mart2; MEF3 like 1; MEF3L; MEF3-like protein 1; myelin expression factor 3-like protein 1; paternally expressed gene 10 protein; retrotransposon gag domain-containing protein 3; retrotransposon Gag like 2; retrotransposon-derived gag-like polyprotein; retrotransposon-derived protein PEG10; RGAG3; RTL2; SIRH1; Sushi-Ichi retrotransposon homolog 1; ty3/Gypsy-like protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.