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Rabbit anti-PEX14 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
PEX14
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 250 and 300
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to PEX14 immobilized on solid support.

The epitope recognized by A303-085A-T maps to a region between residue 250 and 300 of human Peroxisomal Biogenesis Factor 14 using the numbering given in entry NP_004556.1 (GeneID 5195).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to PEX14 immobilized on solid support.

The epitope recognized by A303-085A maps to a region between residue 250 and 300 of human Peroxisomal Biogenesis Factor 14 using the numbering given in entry NP_004556.1 (GeneID 5195).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Peroxisomal biogenesis factor 14 (PEX14) is an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome. [taken from NCBI Entrez Gene (Gene ID: 5195)].

5195
PEX14
peroxisomal biogenesis factor 14
O75381
Peroxisomal membrane protein PEX14

Alternate Names

dJ734G22.2; NAPP2; NF-E2 associated polypeptide 2; PBD13A; peroxin-14; peroxisomal membrane anchor protein PEX14; peroxisomal membrane anchor protein Pex14p; peroxisomal membrane protein PEX14; Pex14p; PTS1 receptor docking protein; PTS1 receptor-docking protein

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.