Rabbit anti-PFAS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-218A-T maps to a region between residue 1 to 50 of human Phosphoribosylformylglycinamidine Synthase using the numbering given in entry NP_036525.1 (GeneID 5198).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PFAS immobilized on solid support.
The epitope recognized by A304-218A maps to a region between residue 1 to 50 of human Phosphoribosylformylglycinamidine Synthase using the numbering given in entry NP_036525.1 (GeneID 5198).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. Phosphoribosylformylglycinamidine synthase (PFAS) catalyzes the fourth step of IMP biosynthesis [taken from NCBI Entrez Gene (Gene ID: 5198)].
Alternate Names
FGAM synthase; FGAMS; FGAR amidotransferase; FGARAT; FGAR-AT; formylglycinamide ribonucleotide amidotransferase; formylglycinamide ribotide amidotransferase; formylglycinamide ribotide synthetase; GATD8; phosphoribosylformylglycinamidine synthase; PURL
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.