Rabbit anti-PHF21A Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
PHF21A (PHD finger protein 21A) is a component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, PHF21A may act as a scaffold [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9Y6X8].
Alternate Names
BHC80; BHC80a; BM-006; BRAF35/HDAC2 complex (80 kDa); BRAF35-HDAC complex protein BHC80; PHD finger protein 21A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.