Rabbit anti-PHIP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-055A maps to a region between residue 1771 and 1821 of human pleckstrin homology domain interacting protein using the numbering given in entry NP_060404.3 (GeneID 55023).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PHIP immobilized on solid support.
The epitope recognized by A302-055A-T maps to a region between residue 1771 and 1821 of human pleckstrin homology domain interacting protein using the numbering given in entry NP_060404.3 (GeneID 55023).
Additional Product Information
PH-interacting protein (PHIP) was isolated as factor that binds to the pleckstrin homology (PH) domain of insulin receptor substrate-1 (IRS-1). PHIP was determined not to be a substrate of the insulin receptor but may instead serve to link IRS-1 to the insulin receptor. PHIP has also been implicated as a positive regulator of beta-cell mitogenesis and survival.
Alternate Names
BRWD2; DCAF14; DDB1 and CUL4 associated factor 14; DIDOD; IRS-1 PH domain-binding protein; ndrp; PH-interacting protein; PHIP; WD repeat-containing protein 11; WDR11
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.