Rabbit anti-PITX1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-577A maps to a region between residues 50 and 100 of human Paired-Like Homeodomain Transcription Factor 1 using the numbering given in entry NP_002644.3 (GeneID 5307).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to PITX1 immobilized on solid support.
The epitope recognized by A300-577A-T maps to a region between residues 50 and 100 of human Paired-Like Homeodomain Transcription Factor 1 using the numbering given in entry NP_002644.3 (GeneID 5307).
Additional Product Information
PITX1 is a homeobox-containing transcription factor involved in the development of many organs. PITX1 has been demonstrated to play a critical role in pituitary organogenesis and can stimulate transcription from most pituitary hormone promoters. PITX1 has also been shown to stimulate the leutinizing hormone (LH)-beta gene promoter and may serve an important role in reproductive gene expression.
Alternate Names
BFT; CCF; hindlimb expressed homeobox protein backfoot; Hindlimb-expressed homeobox protein backfoot; homeobox protein PITX1; LBNBG; paired-like homeodomain transcription factor 1; pituitary homeo box 1; pituitary homeobox 1; pituitary otx-related factor; POTX; PTX1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.